Saturday, March 31, 2007

如果从学术研究和保护的观点看,当年西方探险者带走中国文物的行为,到底是对还是错呢?

不可以否认他们掠夺文物,很大程度上是为了经济利益,然而这也是一些严肃研究的起始——以当时的眼光看,谁可以预料到中国到后来也能有能力进行这些研究呢?而且,现在世界上的一些地区的国家/民族,大约也还没有能力和意愿去严肃地研究他们所占有的文化资源吧,难道我们就应该忽略这些文物?

然而从另一个观点看,又没有人可以预料这些“严肃”研究是否在未来,又不会被推翻而成为事实上的对文物的破坏?

这些西方探险者的行为,既带来了学术的发展,也同时造成了情感的伤害,甚至对研究对象的破坏。紧守现在,则束手束脚;不看未来,则遗祸后人。不如随天而行?

Babel Tower

According to the book I mentioned several days ago, the Babel Tower is based on a tower named E-ur-imin-an-ki ("House of the seven divisions of heaven and earth") localied in the ancient city Borsippa (now Birs Nimrud) adjacent to Babylon. See Page 81-83 for it.
In this way, it seems that the Tanakh is more infulenced by the southern part of the Mesopotamia culture - the Babylonia one.

Report 31-03-07

Everything is done
But I still haven't seen any colony which appears to be correct.
I will try to ligase the Sog_Cen2 structure again tomorrow, with dephosphorylated vector.

ToDo 31-03-07

Induce the expression of transfected DNA
Check the overnight culture

暗暖

天高人渺别离绪
地远缘分聚首难

旅过留痕

Friday, March 30, 2007

Report 20-03-07

Documents posting failed. Okay, I'm shocked by the effiency of FedEx - they still haven't guaranteed my account yet. Right, I am failed to send out the documents, great job!
The lesson is, I should do it in advance. Keep important things done earlier than requested.

Transfection finished, Sog_N1 and Sog_C3 were transfected, Dpp-HA was transfected with them as the control. S2 R+ cells used, they looks fine. (1.8*10exp6 /mL)

Overnight culture purified and digested. The result is not satifsfied, I picked another 16 colonies today and will test them tomorrow.

Western blotting was finished, no expression of Sog-FL found. However, interesting weak bands of a small size appeared in the pellet of Dpp_N and Dpp_C (smaller than Dpp-HA), a band of the same size appeared in the supernatant of Dpp_N as well. I may have a discussion with Hilary about this later.

ToDo 30-03-07

Continue the western blotting
Transfect cells
Send the documents
Purify the plasmid DNA for Sog_Cen2_New, and check by digestion
Reading and writing

monothesim and?

As suggested, there are 3 families of religions across the world: Abrahamic religion, Dharmic religion and Taoic religon.
e...
I want to sleep... Today is not Friday unfortunately - I have to go work tomorrow morning.
Oops...

Thursday, March 29, 2007

Report 29-03-07

Okay, now I can even smell the fish soup in my room - I am sure it is imagination since it is in the kitchen - I just want to eat it now.

Emmm, for today
Bad news, sequencing result recieved and no expected clone is found. Thus I picked another 15 colonies on the plate and seeded them. This is an additional work for today.
All other work except the neutralization for GST-pull down were done.
Western blotting always takes time - this is why I can not finish before 11:30 PM.
The Flag-mad cells seems happy, they tripled in 3 days. S2 R+ cells also reached a incredible density, cells go go go! I will then transfect you tomorrow!
Police Certificate got, I will send it out tomorrow - it is the deadline ordered by my wife. Goddess Bless me.

Hunger is DRIVING Meeeeee

Finally, today's work is finished
I'm empty.. I am coming back, my sweet home, I need food - o, is Carfield here?
No I will not starve to death, I am going now!

In situ pictures

Yes here comes the photos, I am quite happy about them.These embryos are from Vkg mutated, Dcp mutated and Wildtype drsophila melangastor; and the in situ used Race RNA. The embryos photographed were around 3 hours 40 minutes old.

They are shown in the sequence of Wildtype, Vkg mutation, and Dcp mutation.









ToDo 29-03-07

Prepare cells for transfection tomorrow (S2 R+ or/and Flag-Mad)
Write the report
Midi prep
Police certificate
Embryo collecting & fixing (Seed one MT32 collection bottle)
Look for a landline...
Harvest cells (transfected 25-03-07), lyse and do western blot.
Prepare for GST-pull down assay. (Neutralize the protein quantity again?)

靡靡日恍

靡靡日恍,蝇居樊笼,天地不见,吾心不辨,餐兮产兮,宿轮罹阻?

Wednesday, March 28, 2007

Summer night at the top of E'mei mountain

这是一个再普通不过的夏天的夜了
山顶的月光,明亮而冷艳,凉风,或者说,寒风,掠过着地面的一切,人们都缩回了建筑的防御里去,一片静寂,连山崖边铁链的晃动都能听得清清楚楚。
这座山的峰顶如此平缓,更加巧合的是,它的下面就是河流从中穿行而过的冲积平原,沃野千里。城市的灯火,就在脚下闪烁,孤寂千古的山,承载着身躯上好动的人类,却仍怡然自得,在这夏夜里享受它的静谧。
每一个普通的夏天的夜却都如此不同。
满天繁星的神秘,安详大地的自持,我何恃以观?

History, and present

These words from the book "Civilization of Babylonian and Assyrian" make much sense: In studying the past, therefore, in reality studying ourselves, we are concerned with something that is not remote, but on the contrary with something that is quite close to us - with flesh of our flesh and bone of our bone.
The history is a chain, it is not unexpected that philosophers would look for the source of the right of the human spirit in this way. Emmm... How about music?

Fooled myself, feels good!

It seems that I made a conclusion too easily and flippantly, just like a fool.
We can not say that torture and pressure lead to creation - the people lived in the Europe who bear extremely hard environment created no civilization before the influence of Rome Empire reached that area. This kind of pressure lead to another way of evolution - not on the mind but on the body. The fertile land supported big population and then was the birth of language and so on.

Report 28-03-07

All jobs were done.
Used the Fax machine in the general office in the Micheal Smith Building for free -- this is good, for I paid the tution fee.
The USB port only functions when you insert the pen drive directly to the back, not when you install in at the extending cable. This proves the quality of PCs are all the same across the world, so go to hell, the price diffrence!
I will go to the town to get the police certificate tomorrow, I can not wait till Firday.
I hate Mac, it brings no ease to me, moreover, I hate the design of Apple - it's too feminized, I need metal feeling, rough and strong.

ToDo 28-03-07

测序……
准备Midi Prep
收集embryos
发传真
把pen drive带去实验室拷贝照片
明天再去开证明吧- -... 明天老板和其他两个博士要去爱丁堡开会...
这几天挺闲的,顺便多看看书

Tuesday, March 27, 2007

Comic of Hongkong

163的相册终于修好了,网络的盗版人才层出不穷,以前看神兵的相册kiss good bye了,然则新的又冒出了3个。

这港漫确实是神奇的东西,剧情过于单薄,人物塑造真实感欠缺;但是在编排上太有水平了,因此看下去很有娱乐性。

看港漫一定要学习故事编排手法,多线式剧情算是一着;恰到好处的场景变换是另一着;出人意料的情节设计也是必要的。个人性格的设置其实也很有意思,而且很鲜明,不过无限升级的坏风气一定不能学。

Mourn the spirit

If you take a look back to the ancient people, to see what were they mainly concerning. It is easy to find out that in fact they had very similar or should I say the same problems in comparison to us: wife missing husband who is far away, lower layer people complaining about the society, young man loving young woman, old people fearing death and so on...
In the aspect of spirit, there is hardly any progress made by us in thouthands of years. What we are doing now in spirit is just beginning to free it from prejudice, just a beginning.

Introduction to Assyria, is it an introduction anyway?

The rise of Assyria is a good topic to think about, however there are only a few records since this period is too far away from us. Trade took a important part in the conuntry even in this period, though the agriculture is always the most important.
It's worth thinking how the trade and handicraft industry infulence China history, for it is not emphasised in the studies. Some westerners had looked into this field, but they were not going very far for the datas are mostly written in Chinese and not well translated. A good historian is able to see the things at the back of the historic event, like Mao. The detailed work on history like correcting the chronology is not for great historians. It's a pity that the great historians seldom write books on their thoughts, or what they have got from the history is not understood by the people --- severl simple words may mean many things and hide lots of subwords.
Let's get back to Assyria, I can barely understand how the Assyria conquered so many cities and land with such small population. It is likely that the whole Assyria empire is military-based, just like the Mongolian, Xiongnurian(?). In ancient time, agriculture meant almost all economy, as well as army size. I do not konw whether the Assyrians recruit soldiers from the other country (It seems that even they did, the number is limited and small) to support their successively wars, but it is obvious that the wars could not afforded by a Assyrian people. To the east direction, the Assyrians destroyed everything, and never controls the fields which would yield food. Is it because they do not have enough army to maintain the boundary or they just do not need so much food? It seems the Assyrians are quite cruel, and did some massacre, is this making the empire big but weak? And what drives the Assyria Kings to invade the countries nearby is another question. I need more information to explore this part, it is time for me to find some more detailed books.

Here comes one book: http://fax.libs.uga.edu/DS71xJ39C/

Reports 27-03-07

FedEx e-mail received, I will finish and fax the form to their headquarter tomorrow. Thank God I am able to send post safely in the near future --- Alas, my first post with my precious bank statement...

Sequencing result using Sog7 primer on Sog_N1_New is back, looks nice.
Now Sog_N1_New and Sog_C3_new are good, I will start to prepare the plasmid DNA for transfection tomorrow. Thus I will transform these two plasmids today.

Photos of the Race/Ushape marked In situ embryos were taken this morning. I have learnt a lot from Hilary about the different stages of the embryos, she is not a bad teacher though was speaking a little fast --- Okay, I need coffee to listen to her.

Emmm... I really want to paste some photographs here, the embryos looks beautiful. This is a technique problem, I have no idea how to paste it on blogger.

ToDo 27-03-07

今天的ToDo

定拆分Tsg的引物,做Tsg的克隆用。
准备测序样品。
收集果蝇蛋。
Lab meeting。

没什么事儿……看文章吧……

Monday, March 26, 2007

FedEx, give me the mail!

今天忙着做实验,UPS给我打的电话没接到,而我的手机费给老婆打电话用光了,不能回拨,囧。
FedEx的电话倒是接到了,信誓旦旦的跟我说要给我寄e-mail,填好表就能注册好,等到晚上也没看见e-mail的毛……又申请一遍,且看明日如何……
今天没抽出时间去办证,咳咳,明天机会就更差了——今天老板不在……

The Sumerians

学习Sumer历史中……一个比较纯粹的农耕文明
果然逆境造就发展,而且这Sumerian还真是阴沉啊,上天也确实没给他们什么好东西。
公元前9,000年到5,000年的气候湿润期让他们迁徙到mesopotamian,然而5,000 BC之后气候开始干旱,致使其往南部聚集;而最后他们毁于自己造成的土地盐碱化。悲剧的民族。
历史学家给出的他们创造文字和语言的原因是:该地区除了河流域外,全是旱地,故需要大量的水利工程,在此过程中对语言的需求迫切。而他们建立起人类历史最早的城市的原因也在于此:人力需要集中。参照埃及的情况,这种说法还是很有道理的。
不过他们的语言是否人类最古老还不确定,他们的文字记载最早,很大程度上是因为那里的天气适合保存他们的泥板书。换了印度/中国/中美这些地方,早烂光了。
他们的文化,技术,和语言对后来的该地区住民,以及周边的民族影响都非常大,这些人又继续将这些东西传承并继续发展。
果然是苦难导致创造,而安逸让创造浮华吗?

实验室里没有应付Blunt End PCR产物的克隆用连接Kit
为了抑制严重的vector自连
两个方案:
一,把pfu Phusion的PCR产物丢到Taq PCR体系里面去反应一会儿,把Blunt end弄成Sticky end。不过据爱打橄榄球的新西兰人说,这样效率很低。
二,往酶切过后的vector里面加phosphatase,把切口dephosphoralate之。然而据我的经验,这样的连接效率会很低。

总之,都会很低……

事实上在设计实验之前就考虑过这个问题,本来想如果能找出两个可用的内切酶就可以避免,然而这口胡老天却只给我一个可用的。

安吧……大不了我挑100个菌落去测序……反正今天又准备了一堆,正好用来看看酶切出来的电泳结果和序列之间的关系。

顺带一提,上次的4个样品,三个自连,一个连进去,但是方向反了 囧

请大爷你们多下几个蛋吧?

养苍蝇,准确地说是果蝇,还是很令人心烦的。
虽然对你们的基因敲敲补补的责任在于我们,但是,也请大爷你们多下几个蛋吧?
我需要他们来做In situ啊……收集了两个月了也不过150微升,只够做三次……

不过野生型的果蝇太能生了,也是个问题,搞到培养基盖满了蛆,都叠出快1厘米厚了,好在杀果蝇不需要什么证,往bleach里面倒就是了……

Mahler again

真奇了怪了……以前对Mahler一点感觉也没有的,现在怎么突然觉得还不错呢?
号的运用很令人感动,牛;曲子中间的一些快速重复小节我还是很不喜欢;曲子的开头音阶一些小部分不是很合我的意——虽然很新;复调的部分少,但是很精到;传统的上升音阶听起来还是这么舒服;一些配器总在意想不到的时候出现,又在意想不到的时候结束,把整个氛围拔高了一个层次。
Mahler的第五交响,感谢Ovelia共享。

Huge and tiny?

我们这些中国长大的人,对人潮见得太多了,所以有意无意的会忽略个体吧。
伊斯兰教发源,默罕默德举起反旗时的信者也就数千而已,到他死信者也不过数万
数千信众,想必个体的重要性才能得到凸现吧?
而谁知道现在伊斯兰教却是世界第二大宗教,拥有十数亿信徒呢?
时势造英雄确是不假,但其中也隐藏了一些其他东西吧。

ToDo 26-03-07

Monday again!
Today's todo list:
1.Add CuSO4 into the transfected cells to induce the expression. (Sog-FL and Dpp fusion proteins)
2.Check the plasimd DNA.
3.Set sequencing PCR.
4.Send sequencing samples.
5.Paper reading.

I had a good talk with my wife yesterday. I can not understand how the women are thinking, that's really a problem -- women take half of the population. If I can not know clearly the way women thinking, I will lose lots of chances in the future.
It's such a hard work to predict or guess the other people's thoughts, at least as hard as academical work. And it is so important for any one who does not want to stay in his current status.
Lots to learn then, I'm a young man.

Sunday, March 25, 2007

Long live Quake3

Long live the internet! Long live q3acn.com!

虽然我把青春都给了Q3,虽然我的投入从未有所回报,虽然我曾经在q3acn.com存在的证明都已经消失

然而今天我却又下载到了Quake3,在q3acn.com!

一切都不重要了,所有的都可以被宽恕

年少轻狂只有一次,而现在我又摸到它了
我的高中时代,我的大一,我的17岁,我的18岁,我的19岁
我永远的=DOC=Cai-Fan,我永远的Quake3
我亲爱的Q3-tourney2,我亲爱的hunter
我美妙的Rocket,我美妙的Strafe Jump
Rail纷飞,血肉四溅
我,回来了!

ToDo 25-03-07

周日,似乎今天不会下雨。想起上周周日的冰雹,这地方的天气的确有趣得紧。
今天的工作很简单,
1.挑出昨天转的Sog_Cen2_New阳性菌,培养,抽出质粒,然后测序PCR。

2.转染Dpp_N_tm64和Dpp_C_New,用S2R+,昨天观察了下细胞,三天从1*10[6]/ml长到2.4*10[6]/mL,不算非常好,也不算太差,应该能用才是。

3.把昨天作的测序PCR的产物处理了,沉淀DNA,周一拿去送测。

我发现鱼比猪/牛/羊……肉好处理多了,丢进锅里切几片姜和葱一起煮就成,而且味道还可以。
好了,从上周开始,到未来不知道那天为止,我的蛋白质来源就靠你们了,鱼们。

准备练练写文,以我现在这种水准,写什么都不怎么看得下去,所以就拿三国人物开涮吧,这意见不是我提的,不过我觉得不错——反正他们也被涮得够多,火气早涮没了。

周一估计UPS和FedEx要给我打电话,这边的手续弄得还真麻烦,不就寄封信么,又是注册又是确认的,我只想快点儿把信寄出去……而且我的police certification找不着了,难道要周一再花34英镑去重新办一个?这可令人非常不快。